Urea as the best fibrin solubilizer in the thrombin inhibition analysis: Theoretical and experimental modeling of fibrinogen denaturation

Due to worldwide thrombosis incidence, new anticoagulant drugs have been developed. Therefore, diverse techniques are used to measure this capacity. In this sense, a size-exclusion chromatography method has already proposed, which depends on the fibrin solubilization step. Thus, the work aimed to establish the influence of different fibrin solubilizers on fibrinogen denaturation through theoretical-experimental modeling. Theoretical results showed that the coupling energy for fibrinogen-TCA is lower than that obtained for fibrinogen-urea and fibrinogen-bromide coupling, while experimental results evidenced partial fibrinogen precipitation with TCA, NaBr, and urea, resulting this last the best solubilizer to measure thrombin inhibition.